Methods for the Determination of Aloin A and Aloin B in Dietary Supplement Products and Ingredients

December 18, 2015 [Rockville, Maryland] - AOAC INTERNATIONAL invites method authors and developers to submit relevant methods that may meet the AOAC Standard Method Performance Requirements for Standard Method Performance RequirementsSM (SMPR®) for the determination of Aloin A and Aloin B in dietary supplements products and ingredients (AOAC SMPR2015.015).  Submitted methods will be reviewed by an AOAC Expert Review Panel for consideration of AOAC First Action Official Methods status. Methods adopted as AOAC First Action Official Methods by the Expert Review Panel will be published by AOAC INTERNATIONAL.     

The objective of this call for methods is to collect relevant methods that may meet AOAC SMPR2015.015 as approved by the AOAC Stakeholder Panel on Dietary Supplements (SPDS).  All submitted methods will be subjected to evaluation by an AOAC Expert Review Panel, who will review them for potential AOAC First Action Official Methods status. Any resulting approved/adopted Official Methods of Analysis may be used as a reference method.  Acceptable methods must demonstrate that they meet AOAC SMPR2015.015, therefore, being reliable and reproducible when used by trained analysts in accredited laboratories.

Methods must be able to quantitate aloin A and aloin B separately in aloe vera leaf juice and dry juice ingredients, and finished products, as per AOAC SMPR2015.015.  

For the purpose of this Call-for-Methods, aloin A and aloin B are defined as follows:

·         Aloin A:  1,8-Dihydroxy-10-(β-D-glucopyranosyl)-3-(hydroxymethyl)-9(10H)-anthracenone.  Also known as barbaloin.

·         Aloin B:  (10R)-1,8-dihydroxy-3-(hydroxymethyl)-10-[(2S,3R,4R,5S,6R)-3,4,5-trihydroxy-6-hydroxymethyl)oxan-2-yl]-10H-anthracen-9-one. Also known as beta-D-isomer barbaloin or isobarbaloin.

Any analytical technique(s) that measures the analytes of interest and meets the method performance requirements in AOAC SMPR2015.015
will be considered.


AOAC invites method authors to submit their methods with no fee required.  Interested method authors or developers should provide a copy of their proposed method, as well as any available data characterizing the analytical performance and scientific validity of the method.  Ideal method submissions should include a well written method procedure and be accompanied by data or information supporting the method’s applicability and demonstrating the method’s ability to meet the criteria of the AOAC standard method performance requirements. 

Interested method authors looking for guidance on formatting methods and supporting information can find this
here.    The copyright transfer form must also be completed for ALL method submissions.  If the method has been previously published, permission must be obtained from the appropriate parties. Please provide name(s) and complete contact information for the publishing organization and/or corresponding author. 

Please submit your method(s) and supporting data to AOAC INTERNATIONAL by Friday
April 29, 2016 using this form.